Kohli said the "suffering of so many" had left him and Anushka heartbroken.
Must Read: IPL 2020 likely to be cancelled due to COVID-19 crisis; no mega auctions next year
Taking to Twitter, Kohli wrote: "Anushka and I are pledging our support towards PM-CARES Fund & the Chief Minister's Relief Fund (Maharashtra). Our hearts are breaking looking at the suffering of so many & we hope our contribution, in some way, helps easing the pain of our fellow citizens."
As of March 30, the COVID-19 crisis has claimed more than 33,000 lives and infected over 723,000 people globally. In India, around 1100 confirmed cases have been reported while 29 people have lost their lives thus far.
Last week, Kohli and Anushka had urged their fans to stay at home during the nationwide 21-day lockdown, announced by Prime Minister Narendra Modi.
The deadly virus has also brought cricket to a grinding halt as all the series and tournaments have been either cancelled or rescheduled.
Earlier this month, IPL 2020 was rescheduled from March 29 to April 15 due to the epidemic but following the announcement of nationwide 21-day lockdown by the Indian government, the cancellation of cash-rich event now looks inevitable.